DEVELOPMENT OF A SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION OF RUTIN AND QUERCETIN FROM THE FLOWER BUDS OF SOPHORA JAPONICA L.
Abstract
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A simple, rapid, precise, and economical spectrophotometric method for the simultaneous determination of rutin and quercetin isolated from the flower buds of Sophora Japonica L. has been developed. Since rutin and quercetin showed maximum absorbance at 258 and 374 nm, respectively, absorbance was measured at these wavelengths for the determination of rutin and quercetin, respectively. Rutin and quercetin obeyed Beer-Lambert's law in the concentration range of 1-10 µg/ml. The method can be adopted for the routine simultaneous determination of rutin and quercetin.
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References
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